What color is PE fluorescence?
What color is PE fluorescence?
red-orange
R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. It is excited by laser lines from 488 to 561 nm, with absorbance maxima at 496, 546, and 565 nm and a fluorescence emission peak at 578 nm.
What wavelength is Texas red?
Texas Red (and Texas Red-X) Dye. Invitrogen Texas Red dye is a bright red-fluorescent dye with excitation ideally suited to the 561 or 594 nm laser lines. It is commonly conjugated to antibodies and proteins for cellular imaging applications.
What is Texas red staining used for?
Texas Red or sulforhodamine 101 acid chloride is a red fluorescent dye, used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohistochemistry.
Which fluorescent dye can be used for red fluorescence?
Fluorescein and rhodamine dyes are the ones most commonly used to develop biological sensing probes.
What is FITC PE?
The FITC / PE Compensation Standard is to be used in conjunction with hardware or software to remove spectral overlap from fluorochromes into secondary fluorescence detectors of a flow cytometer. Fluorescent signals emitted by fluorochromes can bleed or overlap into the secondary fluorescence detectors.
What color is PE?
Phycoerythrin (PE) is a red protein-pigment complex from the light-harvesting phycobiliprotein family, present in red algae and cryptophytes, accessory to the main chlorophyll pigments responsible for photosynthesis.
What color is Cy3?
The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.
What stains fluorescent dye?
DAPI and Hoechst are widely used blue fluorescent nuclear counterstains. Staining live cells with DAPI requires higher concentration (~10 ug/mL). We offer DAPI dilactate, a more water soluble DAPI salt, which can be used at higher concentrations.
What’s the difference between PE and Texas Red?
Phycoerythrin-Texas Red (PE-Texas Red, also written Texas Red-PE) is a tandem conjugate where PE is coupled to Texas Red dye.
How is the excited fluorochrome related to the Texas Red molecule?
Similar to other tandem conjugates, when excited by 488-nanometer light, the excited fluorochrome (PE) is able to transfer its fluorescent energy to the Texas Red molecule, which then fluoresces at a longer wavelength with a peak in the orange range at 612 nanometers. This tandem is also known by other names such as ECD (Electron Coupled Dye)
What is the absorption spectrum of Texas Red?
Texas Red fluoresces at about 615 nm, and the peak of its absorption spectrum is at 589 nm. The powder is dark purple. Solutions can be excited by a dye laser tuned to 595-605 nm, or less efficiently a krypton laser at 567 nm. The absorption extinction coefficient at 596 nm is about 85,000 M −1 cm −1 .
What kind of laser lines does Texas red dye use?
Invitrogen Texas Red dye is a bright red-fluorescent dye with excitation ideally suited to the 561 or 594 nm laser lines.