What is EpiOcular?
What is EpiOcular?
EpiOcular Eye Irritation Test The EpiOcular EIT is an in vitro hazard assessment assay that has the ability to differentiate materials that are ocular non-irritants from materials that are not ocular irritants. The EIT is applicable to a broad range of organic and inorganic chemicals, and is accepted as OECD TG 492.
What is the eye irritation test?
The Eye Irritation Test (EIT) is an OECD-approved in vitro non-animal test method for identifying chemicals and mixtures that may be irritating to the corneal epithelium.
What is skin irritation test?
The Skin Irritation Test (SIT) is an in vitro, non-animal test designed to identify those chemicals and mixtures capable of inducing moderate skin irritation (UN GHS Category 2 Skin Irritants1), and to discriminate UN GHS Category 2 Skin Irritants from UN GHS 3 Mild Skin Irritants as well as those not requiring …
What is EpiOcular test?
The EpiOcular Eye Irritation Test predicts the acute eye irritation potential of a topically applied chemical or formulation by measurement of its cytotoxic effect of the EpiOcular cornea epithelial model.
What is the Draize eye test used for?
The Draize test is used to measure irritation or corrosion caused to the eye or skin, but it is notoriously unreliable, producing highly variable results. It is also extremely unpleasant and painful, causing eye reddening, swelling, ulceration, even blindness, or skin cracking and bleeding.
What is eye irritancy?
Eye irritation is a general term used to describe the feeling when something is bothering your eyes or the surrounding area. While the symptoms may be similar, there are many possible causes of eye irritation.
What allergens are tested in a skin test?
A skin prick test, also called a puncture or scratch test, checks for immediate allergic reactions to as many as 50 different substances at once. This test is usually done to identify allergies to pollen, mold, pet dander, dust mites and foods. In adults, the test is usually done on the forearm.
Why is the Draize test unreliable?
The Draize test uses rabbits, because they are inexpensive, have large eyes and are easy to handle. However, the rabbit is an inappropriate and inaccurate model of human ocular damage because there are fundamental anatomic differences between the human and rabbit eyelid, tearing mechanism and cornea.
Is the epiocular eye irritation test an in vitro test?
The EpiOcular Eye Irritation Test (EIT) is an in vitro alternative to animal testing and is a validated, OECD test guideline (TG 492). EpiOcular has been used for many years by industry as a non-animal, in vitro alternative to determine Draize scores (for water soluble test articles) and to assess the mildness / ultra-mildness…
How does the epiocular in vitro risk assessment assay work?
EpiOcular MTT ET-50 (Dilution) Similar to the MTT ET-50 Neat method, this in vitro risk assessment assay is based on the effective time at which a material (diluted to 20% in water) causes a 50% reduction in tissue viability (ET-50).
How is the epiocular test system used in science?
Developed more than 20 years ago, EpiOcular has been used for testing thousands of materials. Whether scientists need a risk assessment assay for the irritation potential of an ocular delivered material or the hazard assessment of chemical being transported across the world, EpiOcular is the test system of choice to meet your ocular testing needs.
When did the epiocular eye test come out?
EpiOcular™ has been validated as a Draize Replacement by ECVAM (OECD TG 492 Reconstructed human Cornea-like Epithelium (RhCE) test method) but since its introduction in 1985, EpiOcular™ has been used to determine the ocular irritancy of their products without using animals by many cosmetic,…
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