What is b PER reagent?
What is b PER reagent?
Thermo Scientific B-PER Reagent (in Phosphate Buffer) is an easy-to-use cell lysis reagent is a nonionic detergent-based solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation.
How do you use B per?
Note: The cells can either be used fresh or frozen at -70°C. 2. Resuspend the cells in 10-20 ml of B-PER® Reagent either by vortexing or pipetting up and down until the cell suspension is homogeneous. Once a homogeneous mixture is established, shake it gently at RT for 10 minutes.
What is the purpose of sonication?
Sonication is a process in which sound waves are used to agitate particles in solution. Such disruptions can be used to mix solutions, speed the dissolution of a solid into a liquid (like sugar into water), and remove dissolved gas from liquids.
What can be done with a B-per reagent?
All B-PER Reagents are compatible with downstream applications, such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay).
What is the B-per bacterial protein extraction reagent?
Thermo Scientific B-PER Bacterial Protein Extraction Reagent is a nonionic detergent-based solution that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation.
What is the pH of the B per reagent?
The B-PER Reagent solution contains a proprietary, mild, non-ionic detergent in 20 mM Tris-HCl, pH 7.5. It effectively disrupts cells and solubilizes native or recombinant proteins without denaturation.
Can A B per reagent be used for protease defective expression?
• B-PER Reagent effectively extracts soluble proteins from several common bacterial host strains and is especially suitable for the protease-defective expression host BL21 strains. If lysis is inefficient for a particular bacterial strain, freeze cells before extraction.