What is normal goat serum?
What is normal goat serum?
Normal goat serum (NGS) can be used as an effective blocking agent in a number of immunological techniques since it can successfully reduce nonspecific binding of proteins and antibodies to reaction surfaces and non-specific binding sites. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk.
How long is normal goat serum good for?
This working solution can be stored in the fridge and used for up to one week. Beyond this time, there would be concern for possible microbial contamination that would affect performance.
How do you dilute normal goat serum?
Product Details For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum. Restoration and Storage: Store product at 4°C until opened. Restore with distilled water: 10.0 mL (600 mg package size) and 2.0 mL (120 mg package size).
What is in goat serum?
Description. This 10% Normal Goat Serum blocking solution is ready-to-use, in phosphate buffered saline (pH 7.4) with 0.1% sodium azide. This normal goat serum is intended for use as a blocking solution when a second antibody of goat origin is applied in an immunohistochemical staining procedure.
Why do we use goat serum?
The goat serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample. Serum can be used directly for blocking, or as a constituent of a blocking buffer. Strain: Mixed breed and sex.
What is Bovi sera used for in goats?
Bovi Sera is an absolute MUST HAVE for any goat owner. Useful in the prevention and treatment of conditions such as pneumonia and enterotoxemia, passive immune failure in newborns and shipping fever complex in adults, Bova Sera provides an immediate boost to the immune system allowing goats to get back on their feet.
What is donkey serum?
The donkey serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample. Often the blocking serum is from the species in which the secondary antibody was raised.
What is a blocking agent?
Blocking agents are compounds that inhibit the earliest phase of carcinogenesis through mechanisms that alter drug-metabolizing enzymes, trap cancer-producing compounds that react with activators of carcinogens and oxygen free radicals, and alter rates of DNA repair.
How much is Bovi sera for goats?
Dosage is 2 cc for all goats, regardless of age, sex, weight, or breed. Polyserum or Bovi Sera – Over-the-counter injectable immune system boosters.
How do I use Bovi Sera?
Treatment: Calves – 40-100 ml; Cattle – 75-150 ml; Sheep – 20-40 ml. Administer at 12-24 hour intervals until improvement is noted. Administer IM or subcut. Limit injections to 10 ml per injection site.
What is normal donkey serum used for?
Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.
How do you store donkey serum?
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended.
What can normal goat serum be used for?
Normal goat serum can be used for blocking non-specific binding or as an antibody diluent. I recently purchased Normal Goat Serum, Cat. No. S-1000, from Vector Laboratories for use as a general block in my tissue staining applications.
How is goat serum tested for cross reactivity?
The sera are pooled samples collected from healthy adult goats, horses, chickens, swine, rabbits, cats, and dogs. Each one is heat-treated and centrifuged to remove precipitates and then filtered. The sera are tested with appropriate antibody to check for possible cross-reactivity.
Can a goat be used as a blocking reagent?
Goat Serum, Vector Laboratories. Normal Goat serum can be used as a blocking reagent in Immunoassays. The serum is heat-treated and centrifuged to remove precipitates and then filtered. The sera are supplied undiluted with 0.08% sodium azide as a preservative.
How long is normal goat serum blocking solution good for?
For most IHC and IF tissue staining applications, an aliquot is taken and diluted in assay buffer such as PBS or TBS to 2-10% (v/v). This working solution can be stored in the fridge and used for up to one week.