What does SV40 antigen do?
What does SV40 antigen do?
The large and small tumor antigens (T antigens) are the major regulatory proteins encoded by SV40. Large T antigen is responsible for both viral and cellular transcriptional regulation, virion assembly, viral DNA replication, and alteration of the cell cycle.
How does SV40 promote cellular transformation?
In each case, the SV40-transforming function correlates with the ability of one of the T antigens to bind a cellular protein. Thus, large T antigen binding to the heat shock chaperone, hsc70, the retinoblastoma family (Rb-family) of tumor suppressors, and to the tumor suppressor p53, contribute to transformation.
How does SV40 immortalization work?
SV40-LT is known to inactivate Rb through p53 to promote cell immortalization by preventing cell senescence and apoptosis [7,8,25].
What is SV40 plasmid?
PSF-SV40 – SV40 PROMOTER PLASMID contains the Simian Virus 40 promoter upstream of the multiple cloning site (MCS) for expression in mammalian cells. Transcription termination is mediated by the SV40 poly-adenylation signals downstream of the MCS.
Is SV40 a retrovirus?
SV40 is an abbreviation for simian vacuolating virus 40 or simian virus 40, a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors in animals, but most often persists as a latent infection….
| SV40 | |
|---|---|
| Specialty | Infectious disease |
What is cell immortalization?
For the most part, viral genes achieve immortalization by inactivating the tumor suppressor genes (p53, Rb, and others) that can induce a replicative senescent state in cells. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells.
Is SV40 a plasmid?
SV40 plasmids (vectors) can be packaged only if their DNA is within the range of 3900 to 5300 bp. Since these small genomes do not have much dispensable DNA, it is almost impossible to construct a functional vector with any added genes to it.
Why is SV40 promoter used?
The early promoter of the simian virus 40 (SV40) has been used as a model eukaryotic promoter for the study of DNA sequence elements and cellular factors that are involved in transcriptional control and initiation.
Is SV40 contagious?
Recent molecular biology and epidemiological studies suggest that SV40 may be contagiously transmitted in humans by horizontal infection, independently from the earlier administration of SV40-contaminated vaccines.
What causes polyomavirus?
The human polyomaviruses BKV and JCV are known to cause, respectively, hemorrhagic cystitis in recipients of bone marrow transplantation and progressive multifocal leukoencephalopathy in immunocompromised patients, for example, by HIV infection.
Why do we use CHO cells?
CHO cells are the most common mammalian cell line used for mass production of therapeutic proteins. Products of CHO cells are suitable for human applications, as they allow post-translational modifications to recombinant proteins which can function in humans.
How do you establish a cell line?
The simplest way to create a new cell line is to modify an existing one, a common strategy when an established line already comes close to meeting the requirements. Cells optimized to grow particular viruses or maximize recombinant protein production often come from such modifications.