What are Sf9 cells used for?
What are Sf9 cells used for?
Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus. They were originally established from ovarian tissue. They can be grown in the absence of serum, and can be cultured attached or in suspension.
Do Sf9 cells require serum?
Sf9, Sf21, and High Five™ cells can be grown in serum-required or serum-free media (see Media Considerations, page 8, for more information).
How do you store Sf9 cells?
Store frozen Sf9 cells in liquid nitrogen (vapor phase) until ready to use. Frozen cells are supplied in and may be thawed directly into Sf-900 II SFM. Use the following procedure to thaw cells. Note: We recommend thawing Sf9 cells into suspension culture in shake flasks.
How do you thaw Sf9 cells?
Thawing SF9 cells Remove the supernatant and resuspend the cells in 5-10 ml fresh medium. Seed the entire suspension into a single flask (25-75 cm2) and incubate at 27°C. After 12-24 hrs, remove the old medium and replace with fresh medium. The cells should be dividing within two days.
What are CHO cells and what are they used for?
CHO cells are the most common mammalian cell line used for mass production of therapeutic proteins. They can produce recombinant protein on the scale of 3-10 grams per liter of culture.
Where do Sf9 cells come from?
The Sf9 insect cell line is a clonal isolate derived from the parental Spodoptera frugiperda cell line IPLB-Sf-21-AE, and it is a suitable host for expression of recombinant proteins from baculovirus expression systems (e.g., Invitrogen’s Bac-to-Bac® and Bac-N-Blue™ Expression Systems).
Do insect cells need CO2?
CO2 CO2 exchange is not required for insect cell culture. recommend concentrating the cells by centrifuging them at 100 × g for 5 minutes and resuspending them in fresh medium.
Are Sf9 cells adherent?
The images below show the morphology of healthy Sf9 insect cells in suspension and adherent cultures. Sf9 cells attach firmly to surfaces, and their small, regular size makes them exceptional for the formation of monolayers and plaques.
Are CHO cells cancerous?
CHO cells can also be kept in suspension cultures; in contrast to cancer cells, they are genetically stable; they can be reproduced with expression vectors that contain the “gene of interest” (GOI); they can be transfected; and they remain stable during the process of selection, amplification, single-cell cloning and …
Are CHO cells immortal?
Occasional changes in the genetic makeup of cultured cells allow them to proliferate indefinitely, making them effectively immortal. The most common type of CHO cells has a nutritional requirement for the amino acid proline, which makes this cell line an ideal candidate for genetic studies.
What is the meaning of Sf9?
Sensation Feeling 9
The name SF9 means “Sensation Feeling 9,” in hopes that the boys will shock the world with their talent. The Korean word for nine also sounds like the Chinese character (久) meaning length, which points to the group’s desire to last a long time.
What is the morphology of the Sf9 cell line?
Cell Morphology. The Sf9 insect cell line is a clonal isolate derived from the parental Spodoptera frugiperda cell line IPLB-Sf-21-AE, and it is a suitable host for expression of recombinant proteins from baculovirus expression systems (e.g., Invitrogen’s Bac-to-Bac® and Bac-N-Blue™ Expression Systems).
How are Sf9 cells used in suspension culture?
Sf9 cells express both recombinant GPCRs and G-proteins at much higher levels than mammalian cells. Sf9 cells can be grown in suspension culture, providing an inexpensive way of obtaining large protein amounts. Co-infection with various baculoviruses allows free combination of GPCRs with different G-proteins.
Is the Sf9 cell line a suitable host for baculovirus?
The Sf9 insect cell line is a clonal isolate derived from the parental Spodoptera frugiperda cell line IPLB-Sf-21-AE, and it is a suitable host for expression of recombinant proteins from baculovirus expression systems (e.g., Invitrogen’s Bac-to-Bac® and Bac-N-Blue™ Expression Systems).
How are cells cloned in the Sf9 cell line?
The gene encoding the protein of interest has to be cloned into the multiple cloning site of a pVL-1392 expression plasmid. Sf9 cells are cotransfected with the pVL-1392 plasmid and linearized baculovirus DNA to achieve homologous recombination of both components and production of functional baculoviruses encoding the protein of interest.