What is ATCC cell line?
What is ATCC cell line?
ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development.
Does ATCC test cell lines for HCV?
In addition, ATCC stated “As of January 1, 2010, all human cell lines accessioned into the ATCC general collection are tested for the adventitious agents HIV, Hepatitis B, Hepatitis C, HPV, EBV, and CMV.
What does ATCC stand for in microbiology?
American Type Culture Collection
ATCC (American Type Culture Collection) is a private, nonprofit, global biological resource center and standards organization that provides scientists with the biomaterials and resources they need to conduct critical life science research.
Which benchmark cell line verification is recommended by ATCC?
To standardize STR analysis for human cell line authentication, the American Tissue Culture Collection (ATCC) Standards Development Organization Workgroup has issued standard ASN-0002, which recommends the use of at least eight STR loci (TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317 and D5S818) plus Amelogenin for …
What are common cell lines?
HEK293, or human embryonic kidney-derived epithelial cells, are arguably one of the most widely used cell lines in cell biology research. HEK293 is a rapidly dividing, robust line cell with a good reputation for post-translational modification of its heterologously expressed proteins.
What is BBP certification?
The course teaches staff how bloodborne pathogens are spread, how to avoid exposure and what to do if exposed to infectious material. Those who complete the training receive a Bloodborne Pathogens Training certification valid for one year.
Are human cell lines bsl2?
Cell lines which do not contain known human or animal pathogens are designated BSL – 1. The following list contains human or primate cells that are to be handled using BSL – 2 practices and containment: Cells from blood, lymphoid cells, and neural tissue. Pathogen deliberately introduced or known endogenous contaminant.
What are ATCC strains?
ATCC is the premier source for microbial reference strains. These collections include high-quality, fully authenticated certified reference materials, human microbiome specimens, US National Park Service cultures, and eumycetozoan strains. Our rich portfolio of high-quality materials will move your research forward.
What is ATCC and MTCC?
MTCC and ATCC are simply two different organizations that provide microbial cultures. MTCC is based in India, and ATCC is based in the USA. There are also many others, in many different countries.
How do you verify a cell line?
Verification of the species of a cell line can be performed by probing the sequence associated with species-specific cytochrome c oxidase subunit 1 (CO1), commonly referred to as DNA barcoding [26,27], or by employing PCR assays with other species-specific primers [23].
How do you authenticate cell lines?
The main test for authenticating cell lines is short tandem repeat (STR) profiling, the analysis of microsatellite regions of DNA that have variable numbers of repeats and are located throughout the genome.