What is DR5 GUS?
What is DR5 GUS?
Comments: The auxin-response promoter DR5 consists of 9 inverted repeats of the 11- bp sequence 5′-CCTTTTGTCTC-3′, a 46-bp CaMV35S minimal promoter element, and a TMV leader sequence. Description of accessions available: MT-DR5::GUS is a transgenic plant produced by Dr.
What is DR5 auxin?
DR5 is an artificially synthesized and highly active auxin-response element that contains seven tandem direct repeats of 11 bp, including the auxin responsive factor gene-binding site (TGTCTC) (Ulmasov et al., 1997).
What is DR5 promoter?
A synthetic auxin-responsive promoter called DR5 contains a minimum promoter fused to seven AuxRE repeats (Ul- masov et al. 1997a).
What is DR5 Gfp?
In Arabidopsis, the most widely applied tool is DR5-GFP which uses a synthetic auxin-responsive promoter (DR5rev) to drive the expression of green fluorescent protein (Heisler et al., 2005).
What is DR5 in plants?
Abstract. A highly active synthetic auxin response element (AuxRE), referred to as DR5, was created by performing site-directed mutations in a natural composite AuxRE found in the soybean GH3 promoter. DR5 consisted of tandem direct repeats of 11 bp that included the auxin-responsive TGTCTC element.
Why is the dr5 promoter induced by auxin?
Since the DR5 promoter is induced by auxin, we can infer that the auxin content and responses in the inoculated sites may also be assessed according to the activity of the GUS and YFP genes.
How is auxin detected in a transgenic line?
Auxin can be detected using DR5 -GFP transgenic lines, despite of its indirect connection with auxin abundance in vivo, and the slow time-scale of its auxin induced response (taking several hours from induction) which is not optimized to study fast biological processes such as tropic responses ( Zhao et al., 2014 ).
How is the dr5 reporter system used in Populus?
KEY MESSAGE : Auxin responsive promoter DR5 reporter system is functional in Populus to monitor auxin response in tissues including leaves, roots, and stems. We described the behavior of the DR5::GUS reporter system in stably transformed Populus plants. We found several similarities with Arabidopsis …
What is the role of dr5v2 in Arabidopsis?
DR5v2 is composed of the DR5 promoter and a novel binding site for ARF transcription factors designed to increase sensitivity and precision of auxin response visualization in Arabidopsis ( Liao et al., 2015 ). Rice exhibits divergent morphologies in root, shoot, inflorescence and flower tissue organization compared to dicotyledons.