On which site of pBR322 the tetracycline resistant gene is located?
On which site of pBR322 the tetracycline resistant gene is located?
– BamH 1 site for the restriction enzyme is present on the gene for tetracycline resistance. Pvu 1 and Pst 1 sites are located on the ampicillin resistance gene in pBR 322.
Which restriction enzyme would you cut the pBR322 plasmid vector?
pBR322 contains genes for tetracycline and ampicillin resistance enzymes, as well as sites where it can be cut by specific restriction endonucleases and matching DNA sequences inserted.
Which of these is a marker gene in pBR322?
Complete answer: (a)In the cloning vector pBR322, the selectable markers are ampicillin and tetracycline resistance genes. The role they play within the selection of transformed cells from non-transformed cells is that they support. They also help to differentiate between recombinant cells and non-recombinant cells.
Which type of plasmid is used for transfer of tetracycline resistance gene as a cloning vector?
The pBR322 plasmid is a commonly used cloning vector that contains both the ampicillin and tetracycline resistance genes as selectable markers. Escherichia coli cells transformed with pBR322 plasmids have a selective advantage in antibiotic-treated environments.
Why is pBR322 used as cloning vector?
pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.
What antibiotic resistance genes are in pBR322?
pBR322 is 4361 base pairs in length and has two antibiotic resistance genes – the gene bla encoding the ampicillin resistance (AmpR) protein, and the gene tetA encoding the tetracycline resistance (TetR) protein.
How does pBR322 work as a cloning vector?
The pBR322 plasmid contains a gene that allow the bacteria to be resistant to the antibiotics tetracycline and amipicillin. To use pBR322 plasmid to clone a gene, a restriction endonuclease first cleaves the plasmid at a restriction site. Another plasmid used as a vector to clone DNA is called pUC18 plasmid.
What are the two antibiotic resistance genes in PBR322?
pBR322 is 4361 base pairs in length and has two antibiotic resistance genes – the gene bla encoding the ampicillin resistance (Amp R) protein, and the gene tetA encoding the tetracycline resistance (Tet R) protein. It contains the origin of replication of pMB1, and the rop gene, which encodes a restrictor of plasmid copy number.
What are the characteristics of a pBR322 vector?
The main characteristics of pBR322 are: 1 Restriction sites: BamH I, Hind III, Sal I, Pvu I, Pvu II, Pst I, EcoR I, Cla I 2 Selectable marker: antibiotic resistance genes for ampicillin (amp R) and tetracycline (tet R) 3 ORI: the origin of replication 4 ROP: It codes for proteins, which are involved in the process of replication of plasmid
Are there genes for tetracycline and ampicillin resistance?
PBR322. pBR322 contains genes for tetracycline and ampicillin resistance enzymes, as well as sites where it can be cut by specific restriction endonucleases and matching DNA sequences inserted. From: Biotechnology for Beginners (Second Edition), 2017. Download as PDF. About this page.
Where are the restriction sites on the vector pBR322?
The vector pBR322 was constructed in order to have a plasmid with a single PstI site, located in the ampicillin-resistant gene (Apr), in addition to four unique restriction sites, EcoRI, HindIII, BamHI and SalI.