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What Colour is Alexa Fluor 647?

What Colour is Alexa Fluor 647?

red
Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range.

Is Alexa 647 Cy5?

Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. For stable signal generation, Alexa Fluor 647 dye is generally brighter than Cy5 dye in protein and nucleic acid conjugates. …

Is af647 same as APC?

Alexa Fluor 647 (Ex-Max 650 nm/Em-Max 668 nm): conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC, albeit APC tends to be brighter.

What color is FITC?

FITC emits fluorescence from 475 to 650 nm, peaking at 525 nm, which falls in the green spectrum.

What Alexa 647?

Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. …

How much light does Alexa Fluor 647 absorb?

Alexa Fluor® 647 Technical Information. Alexa Fluor® 647 – conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm.

Is there a single wavelength for red and blue?

Wavelengths of 350nm and below (ultra violet) don’t pass the lens, but if they did we’d have wavelength responding to a colour of red with a tint of blue in it. There would never be a single wavelength for a perfect mixture of red and blue (magenta), as the wavelength that the blue- and red conecells accept the best aren’t multiples of eachother.

What is the wavelength of pure blue 8 bit color?

Pure blue 8 bit color, i.e. RGB (0,0,255), has a quite longer wavelength of 440nm. Since violet of varying degrees extends from 380nm up to ~430nm, you could perhaps refer to 405nm light as “mid violet” in terms of wavelength range.

How is the wavelength of a nanometer displayed?

Wavelength vs. Color Each wavelength in nanometers is displayed in its approximate color By Oguz Yetkinhttp://www.cs.wisc.edu/~yetkin

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What Colour is Alexa Fluor 647?

What Colour is Alexa Fluor 647?

-red
Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range.

How do you dissolve Alexa Fluor?

The Alexa Fluor® NHS ester is typically dissolved in high-quality anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO) (D12345), and the reaction is carried out in 0.1–0.2 M sodium bicarbonate buffer, pH 8.3, at room temperature for 1 hour.

Is Alexa 647 Cy5?

Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. For stable signal generation, Alexa Fluor 647 dye is generally brighter than Cy5 dye in protein and nucleic acid conjugates. …

What does Alexa Fluor 488?

Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

What color is FITC?

green color
FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching.

How long do Alexa Fluor antibodies last?

Storage at 4°C should not exceed 1 or 2 weeks.

What does Alexa Fluor stain?

Alexa Fluor 488 dye dashboard The brightest and most photostable green dye for sensitive detection in live or fixed cells; commonly used with Alexa Fluor 350, 594, and 647 dyes for multiplexing. It is used in stochastic optical reconstruction microscopy (STORM)—as an activator in nSTORM and a reporter in dSTORM.

What Alexa 647?

Alexa Fluor® 647 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor® 647 dye is water soluble and pH-insensitive from pH 4 to pH 10.

Which fluorophores are brightest?

It is concluded that cyanine 3.18 should be useful for multicolor fluorescence experiments and that it may be the brightest fluorophore available for single-color fluorescence immunocytochemistry.

Is Alexa Fluor 488 Green?

Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line.

Is Alexa Fluor 488 FITC?

Molecular Probes™ Alexa Fluor® 488 dye—with nearly identical spectral properties and quantum yield as fluorescein (FITC)—produces brighter, more photostable conjugates that are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection.

Which is the best ester for Alexa Fluor 647?

The NHS ester (or succinimidyl ester) of Alexa Fluor® 647 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH 2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

How are NHS esters used to conjugate Alexa Fluor?

The NHS ester (or succinimidyl ester) of Alexa Fluor® 647 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH 2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Alexa Fluor® conjugate will exhibit brighter…

What kind of dye is Alexa Fluor 488?

Alexa Fluor® 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor® 488 dye is water soluble and pH-insensitive from pH 4 to pH 10.

What is the pH of azdye 647 NHS ester?

AZDye™ 647 NHS Ester is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe. Its excitation ideally suited for the 633 nm or 647 nm laser lines. AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

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