What is a kb DNA?
What is a kb DNA?
A measure of DNA chain length; 1000 nucleotide residues. Tags: Molecular Biology.
What is a standard DNA marker?
The marker is suitable for normal size gels, where DNA fragments will separate within a relatively short distance. For more information on selecting appropriate markers, please visit the Markers and Ladders Selection Guide. Loading.
What is DNA size standard?
Typical size standards are made up of DNA or RNA fragments in variable length in the range of 10bp to 1000bp (base pair) increments. One universally used DNA ladder measures up to 1 kilobase pair (1Kb) and contains 1-10 Kb fragments.
How big is a Neb double stranded DNA marker?
NEB also offers a wide range of double-stranded DNA molecular weight markers for conventional agarose gel electrophoresis. These standards have a size range of approximately 10 bp to 23,000 base pairs.
What is the mass of a Neb DNA ladder?
NEB also offers a Quick-Load version of this ladder with purple dye. Recommended gel percentage range: 0.8-1.2% 1 kb DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel. Mass values are for 0.5 µg/lane.
What is the Quick Load 1 kb DNA ladder?
Quick-Load® 1 kb DNA Ladder is a premixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye. The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis.
How big is a 3.0 kb DNA fragment?
The digested DNA includes fragments ranging from 0.5-10.0 kilobases (kb). The 3.0 kb fragment has increased intensity to serve as a reference band. The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.