What is BspHI?
What is BspHI?
Thermo Scientific PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (isoschizomers: BspHI, CciI, RcaI). Note: Also available as a FastDigest enzyme for rapid DNA digestion.
What is the recognition sequence for PagI?
| Enzyme | Source | Recognition sequence |
|---|---|---|
| PagI | Pseudomonas alcaligenes Sau 14-027 | 5′ TCATGA 3′ AGTACT |
| PalI | Providencia alcalifaciens | 5′ GGCC 3′ CCGG |
| PamI | Phormidium ambiguum | 5′ TGCGCA 3′ ACGCGT |
| PamII | Phormidium ambiguum | 5′ GRCGYC 3′ CYGCRG |
How do you identify an Isoschizomer?
Isoschizomers with alternative cleavage sites are indicated with a ” ^ “. Enzymes that are not currently commercially available are indicated with a ” x “. Neoschizomers are a subset of isoschizomers that recognize the same sequence, but cleave at different positions from the prototype.
What are Isocaudomers explain with suitable examples?
Isocaudomers are restriction endonucleases that produce the same nucleotide extensions but have different recognition sites. The examples are NcoI from Nocardia corallina and PagI from Pseudomonas alcaligenes. They bind different DNA sequences but produce the same sticky ends.
What are isoschizomers and Neoschizomers describe with relevant examples?
Isoschizomers are pairs of restriction enzymes specific to the same recognition sequence. For example, SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers of each other. Neoschizomers are a specific type (subset) of isoschizomer. For example, SmaI (CCC/GGG) and XmaI (C/CCGGG) are neoschizomers of each other.
How does SDS affect the binding of bsphi?
BspHI can remain bound to DNA after cutting and alter migration rate of DNA during electrophoresis. To disrupt binding, add SDS to a final concentration of 0.1%-0.5% or purify DNA before electrophoresis. Impaired by overlapping dam methylation. Why Choose Recombinant Enzymes? What is the molecular weight of BspHI?
What are the reaction conditions for pagi ( bsphi )?
Thermo Scientific PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (isoschizomers: BspHI, CciI, RcaI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
How big is one unit of bsphi enzyme?
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. BspHI can remain bound to DNA after cutting and alter migration rate of DNA during electrophoresis.
Which is E.coli strain carries the bsphi gene?
An E. coli strain that carries the BspHI gene from Bacillus species H (D. Hall). One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. BspHI can remain bound to DNA after cutting and alter migration rate of DNA during electrophoresis.