What is F12 in DMEM?
What is F12 in DMEM?
DMEM/F12 Medium is a 1:1 mixture of Dulbecco’s Modified Essential Medium (DMEM) and Ham’s F-12 Medium, commonly used in growing a variety of mammalian cultures. DMEM/F12 is rich and complex, containing all 21 amino acids, 10 vitamins, glucose, zinc, and iron among other components.
What is composition of DMEM?
The original DMEM formula composition contains 1000 mg/L of glucose and was first reported for culturing embryonic mouse cells. A further alteration with 4500 mg/L glucose has proved to be optimal for the cultivation of certain cell types. View all DMEM products and filter by glucose, L-glutamine, and other components.
How much calcium is in DMEM?
DMEM/Ham’s Nutrient Mixture F-12 (50:50) is frequently used as a base media for the development of proprietary and specialty media to culture CHO cells for biomanufacturing. This media contains 1.05 mM calcium, which is very close to the level of calcium in MCDB media 105 and 110 at 1.0 mM.
Can I use DMEM instead of DMEM F12?
For most cells I could easily substitute the DMEM and F12 as they are very similar is composition. Immune cells however have a tendency to activate in presence of Ca+ ions so DMEM normal glucose is usually best option for them.
Is DMEM F12 serum-free?
Use a base medium that supports serum-free growth. DMEM/F12 is a good base medium for most cell lines. DMEM, MEM and RPMI lack some of the micro-nutrients that are often required for serum-free growth. The F12 portion contains many of the micro-nutrients that cells need for serum-free growth.
Why is DMEM red?
DMEM contains phenol red, which is solely a pH indicator. At physiological pH, the media is a pink-red color. When the media is acidic it turns a yellow-orange color as you describe, and when basic it turns a deep purple (such as if you add bleach).
What is the pH of DMEM?
DMEM (44mM NaHCO3, orange line) is held at physiological pH at CO2 concentrations between 7.5% and 11%. Using DMEM in a 5% CO2 environment will result in a pH of 7.5 which although acceptable for most cell cultures is slightly outside the desired range of physiological pH.
Can I use DMEM F12 instead of DMEM?
For most cells I could easily substitute the DMEM and F12 as they are very similar is composition. F12 contains Ca however so if your cells doesn’t have specific calcium signalling they will most probably behave the same.
Why is DMEM yellow?
Is there calcium in Dmem?
The complete formulation is available. DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium….This DMEM is modified as follows:
| With | Without |
|---|---|
| • High Glucose | • L-glutamine |
| • Phenol Red | • Sodium Pyruvate |
| • HEPES | |
| • Calcium |
Does Dmem contain magnesium?
DMEM media contain increased concentrations of vitamins, minerals, and amino acids compared to traditional basal media. HyClone DMEM media preparations are available in high or low glucose formulations, as well as with or without L-glutamine, magnesium, and sodium pyruvate.
What makes up DMEM-Ham’s F12 cell culture media?
DMEM – Ham’s F12 is a mixture (1:1) of DMEM and Ham’s F12. This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins with Ham’s F12’s wide variety of components.
What kind of cells can DMEM / F-12 support?
DMEM/F-12 is a widely used basal medium for supporting the growth of many different mammalian cells including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
How is DMEM used as a basal medium?
DMEM/F-12 is a widely used basal medium for supporting the growth of many different mammalian cells including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. A 1:1 mixture of DMEM and Ham’s F-12, this formulation combines DMEM’s high concentrations…
Which is GIBCO DMEM / F 12 for cell culture?
Gibco Human Plasma-like Medium (HPLM) is a new formulation designed to resemble the natural cellular environment found in the body, mimicking the metabolic profile of human plasma. Select the right Gibco DMEM/F-12 for your cell culture.