What is FuGENE Transfection Reagents and how does it work?
What is FuGENE Transfection Reagents and how does it work?
FuGENE 6 Transfection Reagent is a nonliposomal, multicomponent reagent proven to efficiently transfect more than 700 cell types. It can be used in the presence or absence of serum, and its minimal cytotoxicity eliminates the need to change medium after transfection.
How does FuGENE work?
FuGENE® HD DNA Transfection FuGENE® HD interacts with nucleic acids to provide efficient and safe entry into the cell, allowing users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells. Its simple, easy-to-use protocol allows users to free up valuable lab time.
What is transient transfection?
In transient transfection, the introduced nucleic acid exists in the cell only for a limited period of time and is not integrated into the genome. However, within a few days most of the foreign DNA is degraded by nucleases or diluted by cell division; after a week, its presence is no longer detected.
Is transfection stable or transient?
Choosing a Transfection Strategy
| Transient Transfection | Stable Transfection |
|---|---|
| Both DNA vectors and RNA can be used for transient transfection. | Only DNA vectors can be used for stable transfection; RNA by itself cannot be stably introduced into cells. |
How does the FuGene 6 transfection reagent protocol work?
FuGENE® 6 Transfection Reagent is a nonliposomal reagent that transfects DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Printed in USA. Revised 4/11.
When to add FuGene 6 to plate cells?
Mix and incubate for 5 minutes. Add an appropriate amount of DNA to FuGENE®6 Transfection Reagent/ medium to achieve the proper ratio of reagent to DNA. Mix and incubate for 15 minutes. Plate cells one day before the transfection experiment so that cells will be approximately 50–80% confluent on the day of transfection.
How much FuGene is needed for dsRNA transfection?
-Always use more FuGENE reagent (µl volume) than RNA (µg mass) -A ratio of 3µl FuGENE to 1-2µg RNA works well in S2 cells -3 uL of FuGENE with 2 uL of dsRNA at a concentration of 1ug/uL is ideal -For co-transfection experiments, if the total amount of RNA exceeds 2µg, increase the amount of FuGENE proportionally.
Where can I buy Promega transfection reagents?
Visit the Transfection Reagents product page to view ordering information for Promega Transfection Reagents. Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods.