What is HT1080 cells?
What is HT1080 cells?
HT1080 (ATCC® CCL-121™) is a human fibrosarcoma cell line initiated from a 35-year-old Caucasian male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases.
What does IMR 90 stand for?
We have investigated the contractile properties and expression of smooth muscle-associated proteins in such a cell line derived from human foetal lung (IMR-90). For almost two decades, these cells have served as model fibroblasts in a wide variety of studies.
Why are U2OS cells used?
The U2OS cell line is extensively used as a circadian rhythm model, including in high-throughput screening studies (Zhang et al., 2009). On the day of HEK293T transfection, U2OS cells were seeded in T25 flasks at 2 × 105 cells/mL, and incubated at 37 °C, 5% CO2 until 70–80% confluence was reached.
What are BJ cells?
The BJ cell line has a long lifespan in comparison with other normal human fibroblast cell lines. These cells have a reported normal diploid karyotype at population doubling 61 but an abnormal karyotype at population doubling 82. They are telomerase negative.
What kind of tumor does HT1080 have?
“Tumorigenicity of human HT1080 fibrosarcoma X normal fibroblast hybrids: chromosome dosage dependency”. Cancer Research. 44 (8): 3471–3479. PMID 6744274.
What do you need to know about the HT1080 cell line?
HT1080 (fibrosarcoma) Description. HT1080 (ATCC® CCL-121™) is a human fibrosarcoma cell line initiated from a 35-year-old Caucasian male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases.
How was the human fibrosarcoma cell line created?
The cell line was created from tissue taken in a biopsy of a fibrosarcoma present in a 35-year-old human male. The patient who supplied the sample had not undergone radio or chemotherapy, making it less likely that unwanted mutations were introduced into the cell line.
What happens to HT1080 cells after implantation?
Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases. *The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection. HT1080 cells are suitable for in vitro and in vivo experimentation.