What is loop-mediated isothermal amplification used for?
What is loop-mediated isothermal amplification used for?
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and rapidity under isothermal conditions. This technique has been used by a number of authors to detect bacterial, parasitic, and viral fish pathogens.
How is loop-mediated isothermal amplification?
Loop-mediated isothermal amplification (LAMP) uses 4-6 primers recognizing 6-8 distinct regions of target DNA. A strand-displacing DNA polymerase initiates synthesis and 2 of the primers form loop structures to facilitate subsequent rounds of amplification.
When was loop-mediated isothermal amplification invented?
20007
A well-established nucleic acid amplification method termed loop-mediated isothermal amplification (LAMP) was first developed by Notomi et al. in 20007. At least four primers, including a pair of outer primers and a pair of inner primers, are used to specifically recognise six distinct DNA sequences on the target gene.
What is LAMP technique?
Loop-mediated isothermal amplification (LAMP) is a single-tube technique for the amplification of DNA and a low-cost alternative to detect certain diseases. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) combines LAMP with a reverse transcription step to allow the detection of RNA.
What is isothermal DNA amplification?
In contrast to PCR, isothermal amplification enables rapid and specific amplification of DNA at constant temperature (60-65 °C) avoiding the requirement of thermal cycling. Phi29 DNA polymerase is the enzyme of choice for whole genome amplification (WGA). …
What is isothermal amplification assay?
Loop-mediated isothermal amplification is a single tube technique for the amplification of DNA. It uses 4-6 primers, which form loop structures to facilitate subsequent rounds of amplification.