What is SMA in immunohistochemistry?
What is SMA in immunohistochemistry?
For In Vitro Diagnostic Use Smooth Muscle Actin (SMA) [E184] is a rabbit monoclonal antibody that is intended for laboratory use in the qualitative identification of smooth muscle actin protein by immunohistochemistry (IHC) in formalin-fixed paraffin-embedded (FFPE) human tissues.
What is IHC protocol?
Immunohistochemistry (or IHC) is a method for demonstrating the presence and location of proteins in tissue sections. Though less sensitive quantitatively than immunoassays such as Western blotting or ELISA, it enables the observation of processes in the context of intact tissue.
What does smooth muscle actin do?
Alpha-smooth muscle actin (SMA), an actin isoform that contributes to cell-generated mechanical tension, is normally restricted to cells of vascular smooth muscle, but SMA can also be expressed in certain non-muscle cells, most notably myofibroblasts.
What is actin a marker for?
Abstract. Beta-actin is commonly used to normalize molecular expression studies due to its high conservation as an endogenous housekeeping gene. However, recent studies have shown that beta-actin expression can change in response to biochemical stimuli, during growth and differentiation, and in disease states.
What is SMA positive?
If your results show a high amount of SMA antibodies, it probably means you have the type 1 form of autoimmune hepatitis. A lower amount may mean you have the type 2 form of the disease. If no SMAs were found, it means your liver symptoms are being caused by something different than autoimmune hepatitis.
What is SMA in a tumor?
In normal human ovaries, alpha-SMA was found in the blood vessel walls, muscle fibers, and stromal cells surrounding the follicles. The main source of alpha-SMA in the benign ovarian tumors was the blood vessel walls which highly expressed the alpha-SMA throughout the tumor.
What are IHC markers?
Immunohistochemical tumor markers are proteins that help doctors tell the difference between different types of cancer. Mesothelioma-related proteins such as calretinin, WT-1 and podoplanin help pathologists differentiate mesothelioma from other cancers such as lung cancer.
Where is smooth muscle actin found?
Alpha-smooth muscle actin, a differentiation marker of smooth muscle cells, is present in microfilamentous bundles of pericytes.
How do smooth muscles contract?
Contraction. Smooth muscle contraction is caused by the sliding of myosin and actin filaments (a sliding filament mechanism) over each other. The energy for this to happen is provided by the hydrolysis of ATP. Unlike cardiac and skeletal muscle, smooth muscle does not contain the calcium-binding protein troponin.
How long can you live with SMA?
Infants with type 1 SMA usually die before their second birthday. Children with type 2 or type 3 SMA may live full lives depending on the severity of symptoms. People who develop SMA during adulthood (type 4) often remain active and enjoy a normal life expectancy.
What kind of antibody is alpha smooth muscle actin?
alpha-Smooth Muscle Actin Antibody Staining Protocol for Immunohistochemistry. Description: ASM-1 represents an excellent marker for myogenic soft tissue tumours and smooth muscle differentiation.
How to stain smooth muscle with IHC World actin?
It reacts specifically witha-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections. Dilution: 1:1000 using IHC-Tek TM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.
What is the control number for smooth muscle actin?
Control Number: 901-305-013015 ISO 9001&13485 CERTIFIED Intended Use: For In Vitro Diagnostic Use SmoothMuscleActin(SMA)[E184]isarabbitmonoclonalantibodythatisintended forlaboratoryuseinthequalitativeidentificationofsmoothmuscleactinproteinby immunohistochemistry (IHC) in formalin-fixed paraffin-embedded (FFPE) human tissues.
What is the antibody staining protocol for ASM-1?
alpha-Smooth Muscle Actin Antibody Staining Protocol for Immunohistochemistry Description: ASM-1 represents an excellent marker for myogenic soft tissue tumours and smooth muscle differentiation.