What type of breast cancer is MDA-MB-231?
What type of breast cancer is MDA-MB-231?
MDA-MB-231 is a highly aggressive, invasive and poorly differentiated triple-negative breast cancer (TNBC) cell line as it lacks oestrogen receptor (ER) and progesterone receptor (PR) expression, as well as HER2 (human epidermal growth factor receptor 2) amplification2,3.
What is a p53 mutation?
Mutations (changes) in the p53 gene may cause cancer cells to grow and spread in the body. These changes have been found in a genetic condition called Li-Fraumeni syndrome and in many types of cancer. The p53 gene is a type of tumor suppressor gene. Also called TP53 gene and tumor protein p53 gene.
Is p53 mutated in HeLa?
Endogenous HeLa p53 proteins are easily detected in HeLa cells transfected with mouse deletion mutant p53 gene. Oncogene.
Is SKBr3 triple negative?
MDA-MB-231 cells are characterized as triple-negative/basal-B mammary carcinoma and SKBr3 are HER2+ mammary carcinoma.
How is p53 inactivated?
In addition to genetic inactivation, the p53 protein can be functionally inactivated in cancer, through post-transductional modifications, changes in cellular compartmentalization, and interactions with other proteins.
Is MCF-7 triple positive?
MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.
Is MCF-7 a TNBC?
MDA-MB-231 is triple negative breast cancer cell lines and they are about 15% to 20% of all breast cancer cases and hard to treat them. Need more research for discovery of new drugs or compounds. MCF-7 is estrogen receptor positive breast cancer cell lines and effective treatments drugs are available.
What is the p53 status of MCF-7, MDA-MB-231?
What is the p53 status of MCF-7, MDA-MB-231, MDA-MB-435 cell lines? What is the p53 status of MCF-7, MDA-MB-231, MDA-MB-435 cell lines? I have to perform experiments with p53 wild type and mutant breast cancer cell lines for which I need to know their p53 status.
Are there cell lines with inconclusive p53 status?
THE NCI-60 PANEL Cell lines with an inconclusive p53 status are shown in red. more information can be found here: Berglind et al., in press: download the manuscript Cell line ATCC number DNA Protein ref consensus comment LYMPHOMA LEUKEMIA CCRF-CEM CCL-119 R248Q 1018 R175H+R248Q Two mutations in separate alleles c.524G>A, c.743G>A R175H+R248Q 3
What kind of cancer can a p53 mutation cause?
Cell lines with a controversial p53 status The NCI 60 panel Bladder cancer cell lines Brain cancer cell lines Breast cancer cell lines Cervical carcinoma Colorectal cancer cell lines Esophageal cancer cell lines Gastric cancer cell lines Head and Neck cancer cell lines Leukemia / Lymphoma cell lines NSCLC cancer cell lines
Is the p53 gene in SK-OV-3 deleted?
In the majority of publications, the p53 status of the SK-OV-3 cell line is stated as “p53 null”. In fact, close examination of the original manuscript shows that the p53 gene in SK-OV-3 is not deleted and did not sustain any gross rearrangement but neither p53 RNA or protein are found.