What is upstream process and downstream process?
What is upstream process and downstream process?
Upstream processes are those in which biological materials are either obtained from an outside source or inoculated and grown in culture, under controlled conditions, to manufacture certain types of products. Downstream processes are those in which the products are harvested, tested, purified and packaged.
What are the steps in upstream processing?
Upstream processing involves all the steps related with inoculum development: Media Preparation, Cell Culture and Cell Separation & Harvest. When the cells have reached the desired density, they are harvested and moved to the downstream section of the bioprocess.
What are the steps involved in downstream processing?
What are the Steps Associated with Downstream Processing?
- Harvest and Filtration.
- Primary Capture.
- Buffer Exchange and Up-concentration.
- Purification (and Contaminant or Impurity Clearance)
- Bioconjugation (Molecule Dependent)
- Formulation.
What are the 3 key steps in bioprocess?
Bioprocesses can be largely classified into three stages: preparation, production and purification.
What is difference between downstream and upstream?
The terms upstream and downstream oil and gas production refer to an oil or gas company’s location in the supply chain. Upstream oil and gas production is conducted by companies who identify, extract, or produce raw materials. Downstream oil and gas production companies are closer to the end user or consumer.
Is fermentation upstream or downstream?
The fermentation or cell culture manufacturing process that is performed to generate the recombinant protein is referred to as the upstream process. Following the completion of upstream manufacturing, the material is transferred for downstream processing. This consists of purifying the recombinant protein.
What is the difference between upstream and downstream?
Is fermentation a downstream process?
Downstream processing involves all unit operations after fermentation that improve the purity of the final ethanol product. Ethanol is typically purified using combinations of distillation and molecular sieving. The fermented broth from the beer well is sent to a traditional distillation column to obtain 95% ethanol.
Which is upstream process?
Upstream processing comprises tasks in the initial stages of the fermentation process in biotechnology. This includes all steps related to the development of microorganisms, nutrient preparation, cell culture, cell separation and harvesting. Upstream processing involves nutrient preparation.
What are downstream activities?
Downstream operations are the processes involved in converting oil and gas into the finished product. These include refining crude oil into gasoline, natural gas liquids, diesel, and a variety of other energy sources.
What is upstream in biotechnology?
The upstream processing in biotechnology involves identifying and extracting the raw materials. This forms the initial process of fermentation. The upstream processing deals with: Inoculum preparation which includes screening or microorganisms and selection of suitable strain and genetic modification of the organism if needed.
What is upstream and downstream processing?
Upstream refers to a stage of production which involves the extraction of raw materials. Upstream refers to any stage of extraction of raw materials. Downstream is the processing stage of the raw materials which can also involve the sale of the goods to the final consumer.
What is upstream process development?
Upstream Process. What is upstream processing (USP)? The upstream part of a bioprocess refers to the initial stage in which microbes/cells are grown, e.g. bacterial or mammalian cell lines in bioreactors. Upstream processing involves all the steps related with inoculum development: Media Preparation, Cell Culture and Cell Separation & Harvest.