What is Capto resin?
What is Capto resin?
Capto resins are based on a highly rigid agarose base matrix that offers outstanding pressure/flow properties, optimized pore structure, and very high chemical stability to support CIP procedures. Capto resins are intended for general use in large-scale bioprocess operations.
How does anion exchange chromatography work?
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). In solution, the resin is coated with positively charged counter-ions (cations).
How does a cation exchange column work?
Ion exchange chromatography separates ions and molecules based on their net overall surface charge. The media in a cation exchange column is negatively charged, binding positively charged molecules, and therefore cations are used for elution of the bound molecules.
What is Capto adhere?
Capto adhere is a multimodal strong anion exchanger for BioProcess applications. It was originally designed for post-protein A purification of MAbs at process scale in flowthrough mode. However, Capto adhere can also be used in bind/elute mode and for applications other than MAbs.
How does Capto adhere work?
Capto adhere is a multimodal anion exchanger. In multimodal chromatography, the ligand interacts with the target molecule through multiple types of interactions. Ionic interactions are commonly involved, but other interactions such as hydrogen bonding and hydrophobic interactions can be significant.
What does anion resin remove?
Cation and anion resins remove dissolved ionic contaminants. Anion IX resins include a strong/weak anion, such as OH- and Cl-. Acting together or alone, these types remove many ionic contaminants from water. Neither type removes non-ionic contaminants such as benzene.
Which of the following is anion exchange resin?
Anion-exchange resin Often these are styrene–divinylbenzene copolymer resins that have quaternary ammonium cations as an integral part of the resin matrix. Reaction: –NR4+OH− + HCl = –NR4+Cl− + H2O. Anion-exchange chromatography makes use of this principle to extract and purify materials from mixtures or solutions.
What is the action of cation-exchange resin?
A cation-exchange resin is a cross-linked polymer with negatively charged structural units. The resin can exchange bound Na+ (Kayexalate) or Ca2+ (calcium resonium) for cations including K+. The purpose of using resins is to enhance the elimination of K+ from the gastrointestinal tract.
Does anion resin lower pH?
Anion resin will remove alkalinity and the pH will be suppressed during the service cycle. pH adjustment may be required.
Is used as anion exchange resin?
What is Capto’s impact chromatography medium used for?
Capto S ImpAct chromatography medium (resin) is a strong cation exchanger (Fig 1). The medium is designed for polishing of monoclonal antibodies (MAbs) and a wide range of other biomolecules. Capto S ImpAct is part of a platform of media based on the Capto product line.
How big is a Capto’s impact bead?
Capto S ImpAct is based on a high-flow agarose base matrix with good pressure/flow properties and an average bead size of 50 μm, which combined with an optimized porosity gives high resolution.
What makes Capto’s impact a strong cation exchange medium?
Capto S ImpAct is a strong cation exchange (CIEX) medium. The medium has a polymer-grafted ligand composed of a mix of two different building blocks, a negatively charged sulfonate group and a neutral pyrrolidone (Fig 2). This composition combines a surface extender with functional groups, which results in a high binding capacity.
What are the benefits of Capto’s impact?
By combining the rigidity of Capto media with an optimized bead, Capto S ImpAct delivers both good pressure/flow properties and good resolution, even at high sample load. The high binding capacity and resolution, combined with the ability to run at high flow rates and bed heights, increase productivity and flexibility in process design.