How do I pack an HPLC column?
How do I pack an HPLC column?
To pack a column, you must:
- • Clean the column and packing device.
- • Prepare the slurry solvent and packing solvent.
- • Prepare the packing device.
- • Prepare your LC system.
- • Assemble the packing device and empty column.
- • Use the backpressure regulator (if appropriate)
- • Position the packing device.
What is L1 packing for HPLC column?
According to the United States Pharmacopeia (USP), L1 column packing is defined as “octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 3 to 10 m in diameter.” Some USP methods specifying an L1 column use intermediate pH, and many of these methods were done on a 10 m, 3.9 x 300 mm column.
What is L19 column?
L19 Strong cation exchange resin consisting of sulfonated, cross-linked styrene divinylbenzene copolymer in the calcium form – about 9 µm in diameter.
What is l11 column?
Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system. Solid and reliable—delivers the peak shape, reproducibility, ruggedness, and performance you demand.
How do you dry pack a column?
In the “dry packing” method, dry silica or alumina is added directly to a column, and solvent is allowed to trickle through in portions, then with pressure. In the “wet packing” method, the column is filled with solvent first, then dry silica or alumina is lightly shaken in, then packed with pressure.
How is a column packed?
A packed column contains particles that either constitute or support the stationary phase, and the mobile phase flows through the channels of the interstitial spaces.
What is phenyl column?
Phenyl columns are first reversed-phase columns, and give retention just as other reversed-phase columns. Next they give specific retention increments for analytes that interact via pi-pi interactions, or a lack of increment for analytes that do not have such specific interactions.
What is L3 packing?
L3 USP columns are described as consisting of porous silica particles, 1.5 to 10 µm in diameter. Our L3 USP columns are produced to the highest quality standards to ensure reliable, reproducible results. A perfect example is: Purospher® STAR Si (5 µm).
What is biphenyl column?
A biphenyl chemistry offers complementary selectivity to a C18 column, and the ability to resolve isomeric compounds, such as drugs of abuse and steroids by taking advantage of π-π interactions between the analyte and the stationary phase.
What is phenyl hexyl column?
Phenyl-Hexyl columns provide unique selectivity for aromatic compounds when compared to other reversed-phase packings such as C18 and C8. Unlike, the standard Phenyl column, Phenyl-Hexyl allows much greater retention and separation of the aromatic compounds through its extended hexyl hydrocarbon functional group.
Why silica is used in HPLC column?
Generally, silica gel is filled in the high-performance liquid chromatography columns because of its particle size and porosity that helps in separation of components and silica gel is also an inert material that does not react with mobile phases. The material filled in the HPLC columns is known as a stationary phase.
Which is USP method for HPLC packing media?
This following listing of HPLC packing media according to USP 621 is intended to assist our customers to find an adequate, commercially available column for implementing a specific USP method. All columns in this list can directly be ordered and obtained via MZ-Analysentechnik GmbH.
How big is a USP L1 column?
It is important to note that, in many cases, USP column specifications are so broad that several (or many) column types actually meet the basic specications. For example, L1 specification calls for a column consisting of silica packing material, 5 or 10µm in diameter, bonded with octadecyl (C18) silane.
What kind of material is used in a L1 column?
For example, L1 specification calls for a column consisting of silica packing material, 5 or 10µm in diameter, bonded with octadecyl (C18) silane. However, a limited number of available C18 columns will actually perform the desired separation.