How do you maintain a Huh7 cell?
How do you maintain a Huh7 cell?
Renew medium 2-3 times a week, or as needed according to media pH, and maintain cell confluency between 30-90%. Do not allow cell concentration to exceed 90% confluency, otherwise cells will decrease proliferation rates. The doubling time of HuH-7 cells is 24 hours.
What is liver cell line?
Liver Cell Lines. To address the issue that in vitro primary human hepatocytes easily lose liver functions and do not proliferate for a long term, several liver cell lines have been developed by gene transfer. Cell lines can be originated from human liver tumors or from immortalized human hepatocytes.
What is smc7721?
Aim: To investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasis, to identify the hepatocarcinoma-specific biomarkers for the early prediction in diagnosis, and to …
What are Huh7 cells?
Huh7 is a type of human liver cell line that may be grown in the laboratory for research purposes. Huh7 cells have been instrumental in hepatitis C research. Until 2005, it was not possible to culture hepatitis C in the laboratory.
What are HepG2 cells?
HepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies. HepG2 cells are nontumorigenic cells with high proliferation rates and an epithelial-like morphology that perform many differentiated hepatic functions.
Why are HepG2 cells used?
HepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies. We further provide protocols for evaluating lipid accumulation, glycogen storage, urea synthesis, and phase I and phase II drug metabolizing activities in HepG2 cells.
What does HepG2 stand for?
Hep G2 (or HepG2) is a human liver cancer cell line. Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma.
Where does the Huh 7 cell line come from?
HuH-7 Origin. HuH-7 is a well differentiated hepatocyte derived cellular carcinoma cell line that was originally taken from a liver tumor in a 57-year-old Japanese male in 1982. The line was established by Nakabayshi, H. and Sato, J. The HuH-7 cell line is an immortal cell line of epithelial-like, tumorigenic cells.
How is Huh-7 cultured as a hepatocyte?
To use as a hepatocyte culture model, Huh-7 was cultured in 3-D Rotating Wall Vessel (RWV) bioreactors engineered by NASA. This is a horizontally rotating cylindrical culture vessel that is meant to grow cells in 3D aggregates, without the shear and turbulence associated with conventional, stirred bioreactors.
How are Huh 7 cells different from HCV cells?
RWV promotes differentiation of the HCV receptive Huh-7 cells. The RWV grown Huh-7 cells are distinct from original Huh-7 cells both morphologically and transcriptionally. In the RWV, these cells form bulky aggregates that are more polarized than the original cells.
How are Huh-7 cells injected into a mouse?
Briefly, exponentially growing Huh-7 cells are injected into the flank of an immunodeficient mouse. The tumors are allowed to grow until reaching a starting size of 150 mm 3 . Upon reaching the starting tumor size, mice are injected with test article according to the study design.