What is NH2 column?
What is NH2 column?
Spherisorb NH2 (Amino) columns contain a silica-based aminopropyl bonded sorbent. The column is listed as USP L8 and is used for anion-exchange, normal-phase and HILIC separations. Part Number: PSS830079. Chemistry. Amino (NH2)
What is inertsil column?
Inertsil ODS columns are general purpose, reversed phase C18 columns available in 5 μm and 10 μm particle sizes. It was the first ODS bonded phase introduced from GL Sciences back in 1986. We recommend InertSustain C18 or Inertsil ODS-4 columns for all new method development.
Why silica is used in HPLC column?
Generally, silica gel is filled in the high-performance liquid chromatography columns because of its particle size and porosity that helps in separation of components and silica gel is also an inert material that does not react with mobile phases. The material filled in the HPLC columns is known as a stationary phase.
How do I clear a blocked HPLC column?
In your case wash the column with 70% water; 15% methanol; 15% acetonitrile. Divert the column eluent to waste not to contaminate your detector(s). Wash the column slowly over to 100% methanol and wash for at least 15 minutes. Wash the column over to 100% acetonitrile and wash for at least 15 minutes.
What is NH2?
NH2 or similar may refer to: Azanide (chemical formula NH − 2) Amino radical (chemical formula NH • 2)
What does Hilic stand for?
Hydrophilic interaction liquid chromatography (HILIC) is an alternative high-performance liquid chromatography (HPLC) mode for separating polar compounds.
How do I flush my HPLC system?
Flush the column with appropriate solvent found in the column manual. Remove and seal column, and store according to good laboratory practice if needed. Flush system with water to remove buffer. Remove all samples from the sampler and store according to good laboratory practice.
Which is better inertsil NH2 or aminopropyl?
Inertsil NH2 shows far superior stability compared to other brand available aminopropyl columns, as our newly developed “Evolved Surface Silica” is chemically bonded with aminopropyl group.
When to equilibrate column and system in HPLC?
If the mobile phase contain buffers or additives, equilibrate the column and system with a mixture of solvents, using the same content as in the buffered mobile phase. For example, if the mobile phase is n-hexane / ethanol / acetic acid (900 /100 / 1), equilibrate the column and system with n-hexane / ethanol (900 / 100) for at least 30 minutes.
Is the GL Sciences HPLC column ISO 9001 compliant?
Thank you for choosing GL Sciences’ HPLC columns. GL Sciences’ HPLC columns are subjected to a rigorous array of QC test in the ISO 9001 compliant facility, with special emphasis on reagent purity, raw material traceability and consistency in raw materials, and final products.
What should the operating pressure be on a HPLC column?
Although the columns are packed by high pressure slurry method, it is recommended to keep the operating pressure under the pressure shown below to maintain peak performance and ensure long column life and stability.